Select or enter the values for your experiment and we will compute the flux values for you.

Reactor Type:
Species:
Nutrient Type:
Oxygen Condition:
Genetic Background:
Growth Rate: (h-1) (normally in the range of [0, 2] h-1)
Total Substrate Uptake Rate: (mmol*g-1*h-1)
Primary Substrate:
Molar Ratio of Primary Substrate: (In the range of [0, 1])
Secondary Substrate: Note: The Molar ratio of the 2nd substrate is calulated as (1 - that of primary substrate) automatically

For genetically modified strain, you can manually set boundaries for fluxes below. Leave intact to use default values. Do NOT enter 0 unless you really want to.

v1: Glucose ⇒ G6P ....................... (glk/ptsG) ...........Min: Max: Default [0, 100]
v2: G6P ⇒ F6P ................................. (pgi) ................Min: Max: Default [-99.9, 99.5]
v3: FBP(F6P) ⇒ GAP + DHAP ....(pfk/fba)...............Min: Max: Default [-51.5, 99.3]
v4: DHAP ⇒ GAP ...........................(tpiA) ................Min: Max: Default [-51.5, 99.3]
v5: GAP ⇒ 3PG ...........................(gapA/gapC)..........Min: Max: Default [-13.5, 216.6]
v6: 3PG ⇒ PEP .............................(gpm/eno) ............Min: Max: Default [-23.3, 196.2]
v7: PEP ⇒ PYR ......................(pyk/ptsG/ppsA).........Min: Max: Default [-36, 232]
v8: PYR ⇒ AceCoA ..........(lpd/pfl/tdcE/aceE/aceF)..Min: Max: Default [-7.9, 213.1]
v9: AceCoA ⇒ Acetate.................. (pta/ackA)...........Min: Max: Default [-144, 135]
v10: G6P ⇒ 6PG ...........................(zwf/pgl)..............Min: Max: Default [0, 151.4]
v11: 6PG ⇒ Ru5P + CO2 .................(gnd).................Min: Max: Default [0, 113.7]
v12: Ru5P ⇒ X5P ............................(rpe)..................Min: Max: Default [-33, 94.1]
v13: Ru5P ⇔ R5P ............................(rpi) .................Min: Max: Default [-94.4, 41.2]
v14: X5P + R5P ⇔ S7P + GAP .......(tkt)..................Min: Max: Default [-2, 47.5]
v15: X5P + E4P ⇔ F6P + GAP .......(tal)...................Min: Max: Default [-6.6, 71]
v16: S7P + GAP ⇒ F6P + E4P........ (tkt)...................Min: Max: Default [-2, 47.5]
v17: AceCoA ⇒ CIT.................. (gltA/prpC).............Min: Max: Default [0, 189]
v18: CIT ⇒ ICIT .............................(acn)...................Min: Max: Default [0, 189]
v19: ICIT ⇒ AKG + CO2 ................(icd)...................Min: Max: Default [0, 189]
v20: AKG ⇒ SUC + CO2 .................(suc)..................Min: Max: Default [0, 194]
v21: SUC ⇒ FUM ...............(sdhABCD/frdABCD)...Min: Max: Default [-105, 194]
v22: FUM ⇔ MAL ....................(fumABC) ..............Min: Max: Default [-106, 194]
v23: MAL ⇔ OAA .....................(mdh/mqo)...............Min: Max: Default [-144.3, 181.5]
v24: ICIT ⇒ GLX + SUC & GLX + AceCoA -->MAL ..(aceA/aceB).........Min: Max: Default [0, 55]
v25: 6PG ⇒ PYR + GAP .............(edd/eda).................Min: Max: Default [0, 148]
v26: AceCoA ⇒Ethanol ..................(adh)...................Min: Max: Default [0, 193.2]
v27: PYR ⇒ Lactate .........................(ldh) ...................Min: Max: Default [-100, 151]
v28: PEP + CO2 ⇔ OAA ..............(ppc/ppk) .............Min: Max: Default [-67.6, 149.8]
v29: MAL ⇒ PYR + CO2 ............ (maeA/maeB)........Min: Max: Default [-13.5, 104.2]

The database used to train this model is here.

This MFlux computing tool is made possible by the team effort of:

This project is supported by NSF under project No.1356669

Full description of MFlux is available in this paper: Wu et al.,Rapid prediction of bacterial heterotrophic fluxomics using machine learning and constraint programming, PLoS Computational Biology, 2016, DOI: 10.1371/journal.pcbi.1004838

If you have any suggestions, corrections or additions of fluxome data, please feel free to contact Dr. Wu (gwu827 at gmail.com), Dr. Bao (forrest.bao at gmail.com) or Dr. Tang (yinjie.tang at seas.wustl.edu)